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. 2023 Nov 3;14:7071. doi: 10.1038/s41467-023-42820-3

Fig. 3. Characterization of IMiD-sensitive proteins and cell viability in primary human T cells and JURKATs.

Fig. 3

A Mass spectrometry proteomics of cell lysates from primary human T-eDHFR-FLAG cells treated with 100 nM 7c for 24 h. Volcano plot shows effect of 7c on protein levels in primary human T-eDHFR-FLAG cells relative to vehicle control. n = 2, each experiment with 4 technical replicates, data point represents mean value. Statistical significance was determined using a two-sided Student’s t test. B Dose–response characterization of off-target binding and degradation of IKZF1 in JURKAT-eDHFR-YFP cells. IKZF1 detected with anti-Ikaros antibody, 53 kDa. COX IV = loading control, 18 kDa. n = 2. C Dose–response characterization of off-target binding and degradation of IKZF3 in JURKAT-eDHFR-YFP cells. IKZF3 detected with anti-Aiolos antibody, 58 kDa. COX IV = loading control, 18 kDa. n = 3. D Dose–response-cell viability characterization of primary human T cells expressing eDHFR-FLAG when treated with 7c for either 24 or 48 h compared to no drug control. n = 1, data points are mean ± SD, 6 technical replicates.