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. 2023 Nov 3;14:7034. doi: 10.1038/s41467-023-42822-1

Fig. 9. Loss of Appl worsens tauopathy in Drosophila.

Fig. 9

a Schematic of crossing scheme to obtain flies expressing wild type human tau in an Applˉ mutant background. Created with BioRender.com. b Locomotor dysfunction in human tau transgenic flies is worsened by removing Appl function as monitored by the climbing assay. p-values for Applˉ vs Applˉ H-tau and H-tau vs Applˉ H-tau are <0.0001. c Quantification shows increased numbers of neurons with caspase activation in whole brains of tau transgenic flies lacking Appl function. p-values for Applˉ vs Applˉ H-tau and H-tau vs Applˉ H-tau are 0.002. d Quantitative analysis shows increased numbers of vacuoles in whole brains of tau transgenic flies lacking Appl function. p-values for Applˉ vs Applˉ H-tau and H-tau vs Applˉ H-tau are <0.0001. Control is nSyb-GAL4/+ in (b, d) and UAS-CD8-PARP-Venus, nSyb-GAL4/+ in (c). **p < 0.01, ****p < 0.0001, one-way ANOVA with Student-Newman-Keuls posthoc test. Data are represented as mean ± SEM. In b, n = 11 repeats per genotype, Control = 84, Applˉ = 109, H-tau = 106, Applˉ H-tau = 75 flies. In c, n = 6 flies per genotype. In d, Control = 6, Applˉ = 6, H-tau = 6, Applˉ H-tau = 7 flies. Flies are 20 days old. Source data are provided as a Source Data file.