Skip to main content
. 2023 Sep 22;10(31):2304632. doi: 10.1002/advs.202304632

Figure 4.

Figure 4

Functional metabolism is altered in mouse embryonic fibroblasts (MEFs) after exposure to polylactide (PLA) degradation products (extract). a,b) Following exposure to amorphous PLA (aPLA; a) or crystalline PLA (cPLA; b) extracts, extracellular acidification rate (ECAR) is increased. c,d) Proton efflux rate (PER) is elevated in MEFs after exposure to aPLA (c) or cPLA (d) extract. e) Bioenergetic levels in MEFs exposed to aPLA or cPLA extracts are decreased in a dose‐dependent manner by 3‐(3‐pyridinyl)−1‐(4‐pyridinyl)−2‐propen‐1‐one (3PO), 2‐deoxyglucose (2DG) and aminooxyacetic acid (a.a.; representative wells are shown). ** p = 0.002, **** p < 0.0001, mean (SD), n = 3 (a–d), n = 5 (e), two‐tailed unpaired t‐test or Brown–Forsythe and Welch ANOVA followed by Dunnett's T3 multiple comparisons test; 100 µl of control or PLA extract was used for 7 days.