Skip to main content
. 2023 Sep 8;10(31):2301120. doi: 10.1002/advs.202301120

Figure 3.

Figure 3

Lysosomal fractionation of SM3 and mHTT‐transfected 293T cells. a) Western blotting analysis of transfected mHTT and SM3 with mEM48 antibody to detect HTT‐N171‐150Q. The blots were also probed with LAMP1 and Vinculin antibodies. b) Quantification of the ratios of mHTT to vinculin (total protein) or LAMP1 (lysosome) on the western blots. The data were obtained from four independent experiments (n = 4). Data were analyzed by two‐tailed Student's t test and presented as mean ± SEM. ***P = 0.0002; ****P <0.0001. c) Western blotting analysis of mHTT, which in transfected 293T cells with SM3 and added proteasome inhibitor MG132 or lysosome inhibitor bafA1. HA transfection was used as control for SM3. d) Quantification of the ratios of mHTT to vinculin on the western blots. The data were obtained from four independent experiments (n = 4). Data were analyzed by one‐way ANOVA with Dunnett's multiple comparisons test and presented as mean ± SEM. ***P = 0.0001; ****P <0.0001. WT293 = un‐transfected 293 cells.