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. 1998 May;64(5):1924–1928. doi: 10.1128/aem.64.5.1924-1928.1998

TABLE 1.

Oligonucleotides used as primers for competitive RT-PCR

Gene 5′ Primer (5′→3′) 3′ Primer (5′→3′)
cbh1-1a AAGATCGTACTAGACGCTAACCG GGTGACAACGGTGAAGGTCTTCGTC
cbh1-2 AAAGTCGTCCTCGACGTGAACTG GGTGACGACAGTGAAGAGTTTGGTG
cbh1-3 AAGATCGTGCTCGACGCGAA GTGACAACGGTGAAGGGCTT
cbh1-4 AAGGTCGTCCTCGACTCGAA CGACGGTGAAGGGCTTGGAG
cbh1-5 ACCGTCGTGCTCGACTCCAA CGACGGTGAAGGGCTTGCTG
cbh1-6 AAGATCGTGCTCGACGCCAA GTGACGACCGTGAACGGCTT
cbh2b AGCGCCAACTACCAGAACTACCT CTTCGGGGTCACGGGGATGAAAC
cdhb CAGTGCGGTGGCATTGGCTG CTGGTCGACGATGAAGGTCG
a

The primers for cbh1-3, cbh1-4, cbh1-5, and cbh1-6 have been described previously (33). The design and relative positions of all cbh1 primers are shown in Fig. 1

b

The cbh2 and cdh primers were based on previously published sequences (23, 32).