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. 2023 Nov 4;22:225. doi: 10.1186/s12934-023-02226-8

Table 1.

Primers used for q-RT PCR for intestinal inflammation and integrity

Primer Forward sequence Reverse sequence
β-actin CCAGTTGGTAACAATGCCATGT GGCTGTATTCCCCTCCATCG
IL-6 AAAGAGTTGTGCAATGGCAATTCT AAGTGCATCATCGTTGTTCATACA
IL-10 CGCAGCTCTAGGAGCATGTG GCTCTTACTGACTGGCATGAG
TNF-ɑ AGTGGTGCCAGCCGATGGGTTGT GCTGAGTTGGTCCCCCTTCTCCAG
IFN-γ AGACAATCAGGCCATCAGCA- AGACAATCAGGCCATCAGCA-
Occludin GCTGTGATGTGTGTGAGCTG GACGGTCTACCTGGAGGAAC
ZO-1 AGGACACCAAAGCATGTGAG GGCATTCCTGCTGGTTACA
PEDV-M GGTTCTATTCCCGTTGATGAGGT AACACAAGAGGCCAAAGTATCCAT