SDS-PAGE (12%) of chloromethane-induced proteins from Methylobacterium sp. strain CM4. Lanes: WT, 10 μg of protein from extracts of wild-type; MA, cells grown on methanol; CM, cells grown on chloromethane; MA/CM, cells grown on methanol and chloromethane; 38A10 and 38G12, 10 μg of protein from extracts of Cmu− mutant cells, i.e., 38A10 (a group 1 mutant) and 38G12 (a group 2 mutant), grown with methanol in the presence of chloromethane. The arrows indicate the 65- and 35-kDa chloromethane-induced proteins.