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Schematics of proteomics workflow deployed in the quantitative analysis of gastric cancer urinary proteins. Urine sample from control individuals (n = 5) and gastric cancer patients (n = 5) was centrifuged and proteins were extracted using 3 KDa centrifugal filter units. Proteins were then enzymatically digested, and peptides were labeled with TMT 10-plex, and fractions were analyzed on mass spectrometer. TMT, tandem mass tags.
