Skip to main content
. Author manuscript; available in PMC: 2023 Nov 5.
Published in final edited form as: Nat Neurosci. 2023 Mar 27;26(5):810–819. doi: 10.1038/s41593-023-01289-5

Extended Data Fig. 3 |. Cholinergic axons respond to auditory stimuli at low intensity and after repeated presentation.

Extended Data Fig. 3 |

a, Mean fluorescence trace of all responsive axon segments for white noise presented at 70 dB SPL (blue), 60 dB SPL (red), and 50 dB SPL (green) (n = 653 axon segments; F(2,156) = 1.51, P = 0.224, one-way ANOVA). Vertical gray line indicates presentation of white noise and shaded region indicates s.e.m. b, Normalized evoked response to white noise, up-sweeps, and down-sweeps at 50–70 dB SPL. (*P < 0.05, **P < 0.01, one-way ANOVA). c, Proportion of axon segments that responded to white noise, up-sweeps, and down-sweeps at 50–70 dB SPL all animals (n = 3). Error bars indicate s.e.m. d, Normalized evoked response to pure tones at 50–70 dB SPL for 3 animals. e, Amplitude of evoked response for white noise across 110 presentations for all animals (n = 3). Faded lines indicate individual animals and shaded region indicates s.e.m. f, Mean evoked response of all responsive axon segments for 1–55 (blue) and 56–110 (red) presentations of white noise (t(54) = 1.00, P = 0.321, two-tailed paired t-test). Vertical gray line indicates presentation of white noise and shaded region indicates s.e.m.