Effects of 264 metabolites on 5-oxo-ETE-induced calcium mobilization in human neutrophils. Intracellular calcium levels were measured in indo-1-loaded neutrophils treated with 264 or one of its synthetic metabolites followed 2 min later by addition of 5-oxo-ETE (10 nM). All compounds are racemic mixtures: 264 (■), ω1-hydroxy-264 (▲, ω-OH); ω-carboxy-264 (△, ωC), dinor-ω-carboxy-264 (▽, dn), ω2-hydroxy-264 (●, ω2-OH), ω2-oxo-264 (○, oxo). Values are means ± SE of data from 4 to 5 independent experiments.