Scheme 1.

(a) Immunogen Retrosynthesis^a and (b) Antibody Generation and Purification Process^b

^aImmunogen 1 was accessed from electrophilic glucosepane precursor 2 and resin-bound peptide 3. ^bThe immunogen was conjugated to keyhole limpet hemocyanin (KLH). Rabbits were immunized, and antiserum was collected. The antiserum was subjected to a series of affinity purifications, and the resulting antibodies were used for all subsequent experiments.