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IC-PCR to detect C. parvum oocysts from estuary samples. Lanes: M, DNA size marker (1-kb ladder), with an arrowhead indicating band of 506 bp; 1 and 2, IC positive controls inoculated with 10 and 100 oocysts, respectively; 3 to 5 and 6 to 8, estuary samples ERS2-2 and ERS1-2, with 500, 50, and 5.0 μl of concentrated sample used per lane, respectively; 9, PCR positive control.
