TABLE 1.
Characteristics and carotenoid contents of the E. coli transformants used in this studya
| Transformant | Plasmid(s) | Carotenoid(s) (mg/g dry wt) | Reference(s) |
|---|---|---|---|
| Part A | |||
| C-1 | pACYC184 | None | 8 |
| ζ | pACCRT-EBP | ζ-Carotene (0.20) | 4 |
| N | pACCRT-EBIRc | Neurosporene (0.29) | 4 |
| L | pACCRT-EBIEu | Lycopene (0.33) | 9 |
| β | pACCAR16ΔcrtX | β-Carotene (0.24) | 6 |
| Z | pACCAR25ΔcrtX | Zeaxanthin (0.29) | 6 |
| Part B | |||
| C-2 | pACYC184 + pUC18 | None | 8, 13 |
| C-2+ | pACYC184 + pKJ21 | None + binding protein | 7, 8 |
| β-h (= β) | pACCAR16ΔcrtX + pUC18 | β-Carotene (0.24) | 6, 13 |
| β-l | pACCRT-EBIEu + pUC-crtY | β-Carotene (0.12) | 9 |
| Z-h (= Z) | pACCAR25ΔcrtX + pUC18 | Zeaxanthin (0.27) | 5, 13 |
| Z-l | pCAR25ΔcrtX + pACYC184 | Zeaxanthin (0.14) | 6, 7 |
| Z-G | pCAR25 + pACYC184 | Zeaxanthin diglucoside (0.30) | 5, 8 |
| Z-P | pACCAR25ΔcrtX + pKJ21 | Zeaxanthin + binding protein (0.28) | 6, 7 |
a
Enzymes encoded by the genes used for construction of the plasmids: crtE, geranylgeranyl pyrophosphate synthase; crtB, phytoene synthase; crtIRc, three-step phytoene desaturase; crtIEu, four-step phytoene desaturase; crtY, lycopene cyclase; crtZ, β-carotene hydroxylase.