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. 2023 Oct 3;24(11):e56614. doi: 10.15252/embr.202256614

Figure EV4. CHX chase analysis of ATAD1 mutant and NS5B.

Figure EV4

  1. 293T cells were transfected with HA‐ATAD1 or ATAD1 domain‐deletion mutants for 24 h, and then the cells were treated with CHX (100 μg/ml) for indicated time duration. The cells were harvested and analyzed by western blotting with anti‐HA antibody.
  2. The ratio of HA:tubulin was analyzed by gel band intensities using ImageJ software. The relative levels of HA‐ATAD1 or ATAD1 domain‐deletion mutant proteins at 0 h were normalized to 1.
  3. WT, ATAD1KO, and ATAD1KO+Res Huh7.5 cells were transfected with Flag‐NS5B for 24 h, and then the cells were treated with CHX (100 μg/ml) for indicated time. The cells were harvested and analyzed by western blotting with anti‐Flag antibody.