Skip to main content
. 2023 Oct 16;24(11):e56845. doi: 10.15252/embr.202356845

Figure 4. Effect of maternal ketogenic diet during PGC formation.

Figure 4

  • A
    A scheme of ketogenic diet feeding experiment on mice.
  • B
    Effects of maternal ketogenic diets on BLIMP1‐expressing nascent PGCs at around E7.0 examined by immunostaining. Rectangular areas shown in the DAPI images correspond to the enlarged images in the insets.
  • C, D
    Quantification of the number of BLIMP1‐positive cells (C), and relative fluorescent intensities of BLIMP1 normalized with DAPI signal in PGCs compared to neighboring epiblasts (D). ES: early‐streak (normal, n = 10; ketogenic, n = 9); MS: mid‐streak (normal, n = 6; ketogenic, n = 10); LS: late streak (normal, n = 7; ketogenic, n = 6) in (C). Total 27–78 cells from three biological replicates in (D).
  • E, F
    Effect of maternal ketogenic diets on the expression of O‐GlcNAcylation in the embryos at around E7.0 examined by immunostaining. Rectangular areas shown in the DAPI images in (E) correspond to (F). The yellow arrowheads in (F) indicate representative BLIMP1‐positive PGCs.
  • G
    Quantification of relative fluorescent signal intensities of O‐GlcNAcylation normalized with DAPI signal in BLIMP1‐expressing PGC and surrounding epiblast cells in the embryos with or without maternal ketogenic diet (Total 46–62 cells from five biological replicates, from late streak to late streak early bud stage).

Data information: Values are plotted as mean ± SE. **P < 0.01, ****P < 0.0001 (Student's t‐test; C, D) and one‐way ANOVA (G). Scale bar: 50 μm, and 25 μm (insets) (B), 250 μm (E), and 75 μm (F). See also Fig EV4.

Source data are available online for this figure.