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. 2023 Nov 6;20:253. doi: 10.1186/s12974-023-02921-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Mertk is efficiently deleted from microglia in the presence of the Cx3Cr1 cre driver A Floxed Mertk mice were generated by the introduction of loxP sites on either side of exon 2 of the Mertk gene. An frt-flanked pGK-neomycin (neo^R) cassette was also introduced for positive selection of embryonic stem cells. A diphtheria toxin A (DTA) cassette was introduced to allow for negative selection. B Representative flow cytometry plot (left) and corresponding histogram (right), indicating Mertk expression in Cx3Cr1^+ve microglia generated from Mertk WT mice (black) compared with Cx3Cr1^+ve microglia derived from Mertk cKO (red). C Widespread Mertk immunopositivity (magenta) is observed in the corpus callosum (dashed lines) of Mertk WT mice following 5 weeks of cuprizone-challenge to induce Mertk expression. Conversely, little to no Mertk expression is observed in the corpus callosum of Mertk cKO mice. Hoechst-labelled nuclei in blue. Scale bar represents 50 µm