TABLE 1.
Bacterial strains and plasmids
| Straina or plasmid | Characteristics | Reference or source |
|---|---|---|
| Strains | ||
| Enterococcus faecalis M1 | Pediocin AcH sensitive | Our isolate |
| Escherichia coli E609L | E609 lpp::Tn10, periplasmic leaky Tcr host for malE-papA plasmids | Henry C. Wu |
| Escherichia coli JM109 | Host for pMBR1.0-type plasmids | 36 |
| Escherichia coli TG1 | Host used for M13 mutagenesis | Amersham |
| Escherichia coli XL1-Blue | Host used for DNA sequencing | Stratagene |
| Lactobacillus plantarum NCDO955 | Pediocin AcH sensitive | 3 |
| Leuconostoc mesenteroides Ly | Pediocin AcH sensitive | Our isolate |
| Listeria innocua Lin11 | Nonpathogenic, pediocin AcH sensitive | Jean Richard, Institut National de la Recherche Agronomique, Paris, France |
| Pediococcus acidilactici LB42 | Pediocin AcH sensitive | Our isolate |
| Pediococcus acidilactici LB42-923 | Pediocin AcH producer | 26 |
| Plasmids | ||
| pMBR1.0 | papABCD operon in pHPS9, Cmr Emr | 4 |
| pMCS9, pMCS14, pMCS24, and pMCS44 | pMBR1.0 cysteine substitution mutant plasmids, Cmr Emr | This study |
| pPR682 | malE plasmid, Apr | New England BioLabs |
| pPR6821 | malE-papA plasmid, Apr | 25 |
| pKN1, pNK5, and so forth | malE-papA substitution mutant plasmids, Apr | This study |
a
The wild-type and mutant MBP-pediocin AcH chimeric proteins were tested against 11 other bacterial strains (gram positive and gram negative) that were either sensitive or insensitive to pediocin AcH. None of the mutants acquired activity against resistant strains.