TABLE 3.

Relative bactericidal activities of wild-type and mutant MBP-pediocin AcH chimeric proteins

Strain	OD600a	Activity units/ml of culture brothb	Protein levelc	Corrected activity units/mld
Wild typee	1.1	1,000	1.0	1,000
K1N	2.2	10	1.4	4
K11E	0.5	1,000	0.8	2,750
W18R	2.3	≤10	1.4	≤3
I26T	2.4	100	1.6	29
M31T	2.3	20	1.6	6
A34D	0.9	≤10	1.8	≤7
N41K	1.0	100	1.1	100
H42L	2.3	400	0.9	212
K43N	1.8	1,000	1.0	611
K43E	2.4	1,000	1.1	417

^aDetermined for culture broths following a 3-h IPTG induction (average of two experiments). 

^bCalculated by (i) determining the minimum volume (microliters) of culture broth required to produce a zone of growth inhibition in a lawn of L. innocua Lin11 cells and (ii) dividing 1,000 AU · μl/ml by this volume (average of four experiments). 

^cRelative protein synthesis levels were determined by laser scanning densitometry of Coomassie brilliant blue-stained gels containing samples of culture broths (average of two experiments). 

^dCorrected for differences in OD₆₀₀ values and relative chimeric protein levels in wild-type and mutant culture broths. 

^eWild-type MBP-pediocin AcH chimeric protein expressed by the E609L/pPR6821 strain.