Fig. 3. Predominant colonization with Staphylococcus is associated with delayed healing time and disturbed microbiome recovery.

A) Survival curves for the healing time for lesions of patients from the M1-M7 clusters combined vs. M0 cluster, as well as separately M6 vs. M0 cluster. Patients who received an alternative form of treatment from an ongoing clinical trial in the clinic were not considered or added to the clinical outcome-related analysis. Log-rank (Mantel-Cox) test was used to calculate the statistical significance, *P=0.02. B) As measured by qPCR, the absolute number of parasites detected in 4 mm punch biopsies from CL lesions taken immediately prior to starting treatment. T-test was used for statistical testing **P<0.01. C) PCoA showing component 1 calculated from weighted UniFrac dissimilarity analysis, including longitudinal swab samples from the M6 cluster (Day0-Day180) and swab samples from the contralateral skin of the same patient. Patients were divided for analysis according to the number of Sb^v rounds required for complete healing (1 vs. >1 round of Sb^v). The connecting lines indicate samples collected from the same patient over time. D) Two representative examples of photographs taken from M0 (Patient #55) and M6 (Patient #54) lesion clusters. The relative abundance pie charts from 16S-seq profiling evaluated over time are indicated on top of the associated photos.