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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Nat Struct Mol Biol. 2023 May 25;30(6):824–833. doi: 10.1038/s41594-023-00991-z

Fig. 5 |. Conserved catalytic core at the 3’ splice site.

Fig. 5 |

a. Cartoon structure of the complex with a box highlighting the 3’ splice site. b. Overview of the three nucleotide bulge in the 3’ splice site with important interactions labeled including TSEN54 (teal) (R36 and K41), TSEN34 (pink) (V284 and K239), and TSEN2 (orange) (R409 and R452). c. Modeled positions of the catalytic residues from TSEN34 (Y247, H255, and K286), which were mutated to alanine to prevent cleavage. d. TSEN2 residues R409 and R452 form a cation-π interaction with the C51 RNA base of the intron to stabilize the bulge. e. The ASL contains the proximal base pair (C32 and G50), important for splicing in vivo. The C32 base forms an A-minor like motif with A53 from the bulge.