Extended Data Fig. 1 |. WT-TSEN cryo-EM processing workflow.

a. A representative micrograph, of curated 9634 micrographs. b. 1,557,097 particles were picked from the 9,634 curated micrographs, 10 representative 2D classes are shown. Particles contained in good classes were used to generate c. ab-initio reconstructions, three of which were selected and further refined using d. heterogenous refinement. The particles contained in the best three classes were further filtered using e. 2D classification and f. heterogeneous refinement prior to two classes being further refined using g. homogenous refinement, with 2x binned particles. h. A final round of heterogenous refinement resulted in one 3D class which was i. refined to an estimated 4.38 Å using homogenous refinement. j. Refinement continued with another round of homogeneous refinement resulting in a resolution of 4. Å following the unbinning of the final 161,512 particles with an estimated resolution of 4.2 Å. k. A local refinement resulted in a final map with an estimated resolution of 3.9 Å.