Extended Data Fig. 2. EndoX-TSEN structure cryo-EM processing workflow.

a. A representative micrograph of 8095 curated micrographs. b. 570,104 particles were picked from the 8059 curated micrographs and used for 2D classification. Particles from the good classes were used to generate c. ab-initio reconstructions, two of which were selected and further refined using d. heterogenous refinement. e. The particles contained in the best class were binned and refined with homogenous refinement. f. The particles were then reextracted and unbinned and used for another round of g. homogenous refinement followed by h. non-uniform refinement and i. local refinement resulting in a final map with an estimated resolution of 3.28 Å. j. FSC of the model to map