Table 1.
Cryo-EM data collection, refinement, and validation statistics
| CLP1-TSEN(AAA)-tRNA-Arg (EMDB-26856) (7UXA) | TSEN(WT)-2’F-tRNA-Arg (EMDB-28755) | ||
|---|---|---|---|
| Data collection and processing | |||
| Magnification | 45000x | 81000x | 81000x |
| Voltage (kV) | 200 | 300 | 300 |
| Electron exposure (e–/Å2) | 54 | 60 | 60 |
| Defocus range (μm) | −0.8 to −1.8 | −1.2 to 2.2 | −1.2 to 2.2 |
| Pixel size (Å) | 0.93 | 0.53 | 0.53 |
| Symmetry imposed | C1 | C1 | |
| Initial particle images (no.) | 570,104 | 1,557,097 | |
| Final particle images (no.) | 153,031 | 161,512 | |
| Map resolution (Å) | 3.32 | 3.93 | |
| FSC threshold | 0.143 | 0.143 | |
| Map resolution range (Å) | 2.8–50.8 | 3.24–60.53 | |
| Refinement | |||
| Initial model used (PDB code) | 6Z9U, 3L0U | ||
| Model resolution (Å) | 3.25 | ||
| FSC threshold | 0.143 | ||
| Model resolution range (Å) | 3.2–3.4 | ||
| Map sharpening B factor (Å2) | 121.9 | ||
| Model composition | |||
| Non-hydrogen atoms | 8120 | ||
| Protein residues | 820 | ||
| Nucleotides | 78 | ||
| Ligands | 2 (Mg) | ||
| B factors (Å2) | |||
| Protein | 76.24 | ||
| Nucleotide | 81.90 | ||
| Ligand | 22.87 | ||
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.004 (0) | ||
| Bond angles (°) | 0.832 (0) | ||
| Validation | |||
| MolProbity score | 1.77 | ||
| Clashscore | 7.47 | ||
| Poor rotamers (%) | 0.88 | ||
| Ramachandran plot | |||
| Favored (%) | 94.78 | ||
| Allowed (%) | 5.22 | ||
| Disallowed (%) | 0.00 | ||