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GalK, GalT, and GalE activities encoded by pKBL275 in the Δgal mutant S165 of E. coli K-12 and in control strains
| Strain/plasmid | Activity (nmol/mg/min) of:
|
||
|---|---|---|---|
| GalK | GalT | GalE | |
| L. casei 64H (Gal+) | 83 | 115 | 900 |
| E. coli DH5α (Gal+) | 24 | 57 | 1,400 |
| S165/pSU19 | 0 | 0 | 0 |
| S165/pNP5 (galKTM′) | 45 | 448 | NDa |
| S165/pKBL275b (galKETRM′) | 66 | 44 | 900 |
ND not determined.
pKBL275 is a pSU19 derivative carrying the insert of pKBL1.
