TABLE 3.
GalK, GalE, and GalT activities in L. casei 64Ha
Carbohydrate | Activity, [nmol/mg/min] (relative activity) of:
|
||
---|---|---|---|
GalK | GalE | GalT | |
None | 17.5 (1.0) | 230 (1.0) | 7.3 (1.0) |
Galactose | 150.0 (8.5) | 2,500 (10.9) | 71.3 (9.8) |
Lactose | 18.9 (1.1) | 167 (0.7) | 6.3 (0.9) |
Glucose | 7.6 (0.4) | 250 (1.1) | 4.7 (0.6) |
Extracts were prepared as described in Materials and Methods. Cells were grown in LCM alone or in LCM with galactose, lactose, or glucose for 3 h prior to harvesting.