Table I. List of Abs used for flow cytometric analysis of B1-8i cells pretransfer.
| Abs Used in Stains | Company and Clone | Identifier | Dilution |
|---|---|---|---|
| Abs used in surface stain | |||
| BUV563-coupled anti-mouse CXCR4 | BD (2B11/CXCR4) | 741313 | 1:400 |
| BV480-coupled anti-mouse IgG1 | BD (A85-1) | 746811 | 1:500 |
| BV650-coupled anti-mouse CXCR3 | BioLegend (CXCR3-173) | 126531 | 1:500 |
| BV785-coupled anti-mouse CXCR5 | BioLegend (L138D7) | 145523 | 1:200 |
| PE-coupled NP | Biosearch Technologies | N-5070-1 | 1:100 |
| PE-Cy7-coupled anti-mouse CD40 | BioLegend (3/23) | 124622 | 1:2000 |
| A700-coupled anti-mouse MHCII IA/IE | Invitrogen (M5/114.15.2) | 56-5321-82 | 1:2000 |
| ViaKrome808-coupled Live/Dead | Beckman Coulter | C36628 | 1:2000 |
| APC-Fire750-coupled anti-mouse CD86 | BioLegend (GL-1) | 105045 | 1:1000 |
| Abs used in intracellular stain | |||
| BUV395-coupled anti-mouse CD11c | BD (N418) | 744180 | 1:1000 |
| BUV496-coupled anti-mouse CD23 | BD (B3B4) | 741058 | 1:2000 |
| BUV661-coupled anti-mouse CD19 | BD (1D3) | 612971 | 1:2000 |
| BUV737-coupled anti-mouse CD62L | BD (MEL-14) | 612833 | 1:1000 |
| Pacific Blue-coupled IRF4 | BioLegend (IRF4.3E4) | 646418 | 1:1000 |
| BV510-coupled anti-mouse CD21 | BD (7G6) | 747764 | 1:2000 |
| AF488-coupled anti-mouse GL7 | Invitrogen (GL-7) | 53-5902-82 | 1:1000 |
| Spark YG593-coupled anti-mouse CD11b | BioLegend (M1/70) | 101282 | 1:2000 |
| PE-Fire 640-coupled anti-mouse CD38 | BioLegend (90) | 102744 | 1:2000 |
| Spark NIR685-coupled anti-mouse IgD | BioLegend (11-26c.2a) | 405750 | 1:2000 |
| APCFire810-coupled anti-mouse B220 | BioLegend (RA3-6B2) | 103278 | 1:1000 |
AF, Alexa Fluor; APC, allophycocyanin.