Table II. List of Abs used for flow cytometric analysis of mouse LN cells postimmunization.
| Abs | Company and Clone | Identifier | Dilution |
|---|---|---|---|
| BUV661-coupled anti-mouse CD19 | BD (1D3) | 565076 | 1:2000 |
| BUV737-coupled anti-mouse CD4 | BD (RM4-5) | 612843 | 1:500 |
| BV480-coupled anti-mouse IgG1 | BD (A85-1) | 746811 | 1:500 |
| BV510-coupled anti-mouse CD95 | BD (Jo2) | 563646 | 1:200 |
| BV605-coupled anti-mouse CD86 | BioLegend (GL-1) | 105037 | 1:200 |
| BV711-coupled anti-mouse CD138 | BioLegend (281-2) | 142519 | 1:500 |
| Alexa Fluor 532–coupled IgM | Conjugated in-house (mAb from Thermo Fisher Scientific, clone II/41) | 1:500 | |
| PerCPCy5.5-coupled anti-mouse CD38 | BioLegend (90) | 102722 | 1:400 |
| PE-coupled NP | Biosearch Technologies | N-5070-1 | 1:100 |
| PE eFluor610-coupled anti-mouse CD45.1 | Invitrogen (A20) | 61-0453-82 | 1:200 |
| PE-Cy7-coupled anti-mouse GL-7 | BioLegend (GL-7) | 144620 | 1:200 |
| APC-coupled CXCR4 | BioLegend (L276F12) | 146508 | 1:400 |
| Spark NIR685-coupled anti-mouse IgD | BioLegend (11-26c.2a) | 405750 | 1:1000 |
| APCFire810-coupled anti-mouse B220 | BioLegend (RA3-6B2) | 103278 | 1:500 |
| A700-coupled anti-mouse CD45.2 | BioLegend (104) | 109822 | 1:400 |
| APC-e780-coupled Live/Dead | eBioscience | 65-0865-14 | 1:10,000 |
APC, allophycocyanin.