Table III. List of Abs used for FACS of mouse cells.
Abs | Company and Clone | Identifier | Dilution |
---|---|---|---|
BUV661-coupled anti-mouse CD19 | BD (1D3) | 565076 | 1:500 |
BV510-coupled anti-mouse CD45.2 | BioLegend (104) | 109838 | 1:500 |
BV605-coupled anti-mouse IgG1 | BD (A85-1) | 563285 | 1:500 |
BV785-coupled anti-mouse B220 | BioLegend (RA3-6B2) | 103246 | 1:500 |
PerCPCy5.5-coupled anti-mouse CD38 | BioLegend (90) | 102722 | 1:500 |
PE-coupled NP | Biosearch Technologies | N-5070-1 | 1:100 |
PE-Cy7-coupled anti-mouse GL-7 | BioLegend (GL-7) | 144620 | 1:500 |
APC-coupled anti-mouse CD3 | BioLegend (17A2) | 100236 | 1:500 |
APC-coupled anti-mouse CD4 | eBioscience (GK1.5) | 17-0041-83 | 1:500 |
APC-coupled anti-mouse CD11c | BioLegend (N418) | 117310 | 1:500 |
APC-coupled anti-mouse Ly6c | Invitrogen (HK1.4) | 17-5932-80 | 1:500 |
A700-coupled anti-mouse CD45.1 | BioLegend (A20) | 110724 | 1:500 |
APC-e780-coupled Live/Dead | eBioscience | 65-0865-14 | 1:10,000 |
APC, allophycocyanin.