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. Author manuscript; available in PMC: 2023 Nov 5.
Published in final edited form as: Cell Rep. 2023 Jul 30;42(8):112861. doi: 10.1016/j.celrep.2023.112861

Figure 3. Avirulent C. difficile strain demonstrates no virulence in innate immune-deficient mice and germ-free mice.

Figure 3.

(A–D) MyD88−/− mice (n = 4 per group) were treated with MNV and clindamycin before being orally administered with 200 spores of C. difficile strains. Daily body weight and acute disease scores were monitored for 7 days post-infection.

(A) Percentage of weight loss to baseline of mice infected with indicated strains.

(B) Acute disease scores comprising weight loss, body temperature drop, diarrhea, and morbidity of mice infected with indicated strains.

(C) Fecal colony-forming units measured by plating on selective agar 1 day post-infection.

(D) Fecal Tcd toxins measured by CHO cell rounding assay 1 day post-infection.

(E–H) Germ-free mice (n = 3 to 5) orally administered with 200 spores of indicated C. difficile strains. Daily body weight and acute disease scores were monitored for 10 days post-infection.

(E) Percentage of weight loss to baseline of mice infected with indicated strains.

(F) Diarrhea scores of mice infected with indicated strains 2 days post-infection. Results represent min to max showing all points.

(G) Fecal colony-forming units measured by plating on selective agar at 6, 12, and 24 h post-infection.

(H) Fecal Tcd toxins measured by CHO cell rounding assay at 6, 12, and 24 h post-infection. Results represent means ± SD. *p < 0.05, **p < 0.01, ****p < 0.0001.