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. 2023 Nov 6;7(1):e202302335. doi: 10.26508/lsa.202302335

Figure 8. ESYT1 regulates mitochondrial lipid homeostasis.

Figure 8.

Sucrose bilayer purified mitochondria from control human fibroblasts (control, n = 3), ESYT1 KO fibroblasts (KO, n = 4) and ESYT1 KO fibroblasts expressing either ESYT1–Myc (Rescue, n = 6) or an mitochondria–ER artificial tether (Tether, n = 6) were analyzed for absolute quantification of lipid content using shotgun mass spectrometry lipidomics. (A) PCA analysis of individual samples. Lipid species mol% were used as input data. (B) Lipid class profile of cardiolipins (CL), phosphatidylethanolamines (PE), phosphatidylinositols (PI), and phosphatidylcholines (PC). Data are presented as molar % of the total lipid amount (mol%). One-way ANOVA with multiple comparisons analysis was applied. Error bars represent mean ± SEM. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.