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. 2023 Nov 6;7(1):e202302335. doi: 10.26508/lsa.202302335

Figure S2. SYNJ2BP effect on MERCs is independent of the mitochondrial fission-fusion machinery.

Figure S2.

(A) Confocal microscopy images of control human fibroblasts and fibroblasts overexpressing SYNJ2BP. Top panel: SYNJ2BP localization (green), cytochrome C (CytC) serves as a mitochondrial marker (cyan) and KDEL as an ER marker (magenta). Bottom panel: ESYT1 localization (green), CytC serves as a mitochondrial marker (cyan), and KDEL as an ER marker (magenta). White arrowheads highlight the accumulation of endogenous ESYT1 at MERCs when SYNJ2BP is overexpressed. Scale bar = 10 μm. Zoomed-in images, scale bar = 2 μm. (B) Confocal microscopy images of human fibroblasts. CytC serves as a mitochondrial marker (green), HSPA5 as an ER marker (magenta), and nuclei are stained with DAPI (blue). (a, b) Knock-down of DRP1 in control fibroblasts (a) and in SYNJ2BP overexpressing fibroblasts (b). (c, d) Knock-down of MFN2 in control fibroblasts (c) and in fibroblasts overexpressing SYNJ2BP (d). Scale bar = 10 μm.