FIGURE 6.

Neuron‐derived EVs activate the mTOR/Akt survival signalling via the phosphorylation of Ser473 residue of Akt and Ser235/236 residues of RPS6. (a) Schematic representation of the experimental procedure. Cultured cortical neurons were exposed to 6‐OHDA 50 µM or not (Ø) and 16 h later, EVs were isolated from the cultured medium and were administered to target cortical neurons at a dose of 400 EVs:cell. After 4, 16, or 24 h of treatment, cell extracts were collected and subjected to WB. (b) Representative immunoblots show phosho‐Ser473‐Akt, phospho‐Ser235/236‐RPS6, total Akt, total RPS6, and actin as a loading control. (c) Densitometric analysis of phospho‐Ser473‐Akt levels and phospho‐Ser235/246‐RPS6 levels. Values represent culture replicates of at least three independent neuronal cultures (mean ± SEM). Data were analysed by One‐way ANOVA with Bonferroni's multiple comparison test (***P < 0.001 vs UT and ##P < 0.01).