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. 2023 Oct 12;8(11):2020–2032. doi: 10.1038/s41564-023-01496-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — (a) Parasites and host cells isolated from the AT were differentiated based on Pacific Blue (Hoechst 33342) intensity and stumpy forms of the parasite were identified based on positive FITC (GFP::PAD1_3’UTR) signal. (b) Parasites were selected based on positive PerCP (propidium iodide) signal. Cell cycle was analysed in the entire parasite population or within FITC (GFP::PAD1_3’UTR) positive and negative populations. Cell cycle stages were attributed based on PerCP (propidium iodide) signal distribution. (c) 3T3-L1 pre-adipocytes, splenocytes and parasites were identified based on SSC-A vs FSC-A gating. Non-viable cells were identified based on positive propidium iodide signal.