Fig. 2. Induced adipocyte lipolysis is independent of mice feeding behaviour and dependent on adaptive immune response.
a, Release of FFAs from gonadal AT explants of infected and non-infected WT mice under fasted and re-fed conditions, normalized to non-infected mice. For days 0 and 6–16, n = 10 and 6 mice, respectively, were examined in a single experiment. b, Daily food intake normalized to non-infected ad libitum-fed mice. For days 0–6 and 7–8, n = 15 and five mice were examined per group over two independent experiments. c, Release of FFAs from gonadal AT explants of infected and non-infected WT mice, normalized to ad libitum-fed non-infected mice. n = 9 mice for non-infected ad libitum group, and n = 10 for infected and non-infected pair-fed groups, examined over two independent experiments. d, Representative parasitaemia of infected WT and Rag2−/− mice. n = 8 WT and 7 Rag2−/− mice examined in a single experiment. e, Fat mass relative to baseline of infected Rag2−/− and WT controls. n = 6 WT and 7 Rag2−/− mice examined over two independent experiments. f, Release of FFAs from gonadal AT explants of infected WT and Rag2−/− mice normalized to non-infected WT controls. n = 11 non-infected WT mice, 11 infected WT mice and 9 infected Rag2−/− mice examined over two independent experiments. Error bars represent the s.e.m. Statistical analysis was performed with one-way (a, c and f) and two-way (b, d and e) ANOVA using Šidák’s test for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Statistical source data contain additional parameters.