Fig. 3.

Immune cell alterations revert after cessation of CSF1R inhibition. (a) Schematic illustration of the experimental set-up in female wild-type mice fed a PLX5622-containing diet or control diet starting at 6–7 weeks of age. After 4 weeks, all mice received control diet. (b–f) Frequency of microglia in brain (b), macrophages in the peritoneum (c), M2 (resident) macrophages, M1 (inflammatory) macrophages and eosinophils in adipose tissue (d), C-C motif chemokine receptor 2 (CCR2)⁻ (resident) macrophages, CCR2⁺ (inflammatory) macrophages, eosinophils and DCs in the colon (e) and liver macrophages (f) in mice originally fed a PLX5622-containing diet (purple circles) or control diet (green triangles). Data are shown as mean±SEM of one experiment with four mice each experiment; each data point represents an individual mouse. AT, adipose tissue; ATM, adipose tissue macrophages; Lin, lineage (‘dump channel’, including CD3-, CD19-, Ly6G- and NK1.1-specific antibodies to exclude T and B cells, granulocytes and natural killer cells); PLX, PLX5622. *p<0.05, analysed by unpaired Mann–Whitney U test with two-tailed distribution