Fig. 6.

Macrophage depletion following CSF1R inhibition leads to impaired beta cell function. Male wild-type mice were fed a PLX5622-containing diet (purple circles) or control diet (green triangles) from 4–5 weeks of age onwards, for up to 8 months. (a, b) Ex vivo GSIS (a) and stimulation index (b) after 5.5 months of PLX5622 treatment. (c, d) In vivo insulin secretion upon glucose stimulation after 3 months (c) and 8 months (d) of treatment, comparing control with PLX5622-treated mice. (e–h) Islet macrophages (e, g) and insulin secretion (f, h) in female mice upon fasting and refeeding after 4 weeks on (e, f) and 4 weeks off (g, h) PLX5622 treatment. (i–l) Alpha/beta cell ratio (i), beta cell mass (j), number of islets (k) and size distribution (small, 10–1000 µm²; medium, 1000–10,000 µm²; large, 10,000–100,000 µm²) (l). Data are shown as mean±SEM and representative of three (a, b), two (c, d, i–l) or one independent experiment(s) (e–h). For all graphs showing single data points (a, b, e–h, j–l), each data point represents an individual mouse. PLX, PLX5622. *p<0.05, **p<0.01, unpaired Mann–Whitney U test with two-tailed distribution