FIG. 3.

Effect of removal of 3-methylbenzoate from the culture medium of P. putida EEZ32 and the contained strain P. putida CMC4. Cells of P. putida EEZ32 (▪ and □) and P. putida CMC4 (• and ○) growing exponentially on M9 minimal medium containing glucose and 15 mM 3-methylbenzoate were filtered, thoroughly washed with 50 mM phosphate buffer, and resuspended in M9 minimal medium containing glucose but not 3-methylbenzoate (□ and ○) or in M9 minimal medium containing glucose and 15 mM 3-methylbenzoate (▪ and •). At the times indicated the numbers of viable cells (CFU per milliliter) were determined in triplicate in LB medium supplemented with 5 mM 3-methylbenzoate and appropriate antibiotics.