TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| P. putida strains | ||
| CMC4 | (pWW0), Kmr 3MB+ mini-Tn5/ Km::(xylS Pm::lacI Plac::gef) | This study |
| CMC5 | Kmr, pCC102, mini-Tn5/ Tc::Pm::′luxAB | This study |
| EEZ32 | (pWW0), pCC102, Kmr Tcr 3MB+ | 26 |
| KT2440 | Prototroph, Cmr | 8 |
| UWC1 | Rifr derivative of KT2440 | 23 |
| E. coli strains | ||
| CC118λpir | Rifr, host for pSM1350 | 9 |
| HB101 | Smr, host for pRK600 | 13 |
| Plasmids | ||
| pCC102 | Kmr, xylS2 Pm::lacI | 5 |
| pRK600 | Cmr, ColE1 oriV, RK2mob+tra+ | 13 |
| pSM1350 | Kmr, mini-Tn5, xylS Pm::lacI, Plac::gef | This study |
| pWW0 | IncP9, mob+ tra+ 3MB+ | 21 |
a
Kmr, Apr, Cmr, and Rifr, resistance to kanamycin, ampicillin, chloramphenicol, and rifampin, respectively; 3MB+, ability to grow on 3-methylbenzoate.