Fig. 2.

Removal of TA, a pseudo-vitreous cortex, with the Beriplast P. Freeze-dried product A (human fibrinogen, 240 mg; human factor XIII, 180 IU; bovine aprotinin, 3000 KIE) and freeze-dried product B (human thrombin, 900 U) were reconstituted in 3 mL of BSS, solution A and B (undiluted), respectively. Besides, BBG was added to solution A to give a final concentration of 270 µg/mL. A TA layer. B The Beriplast P hydrogel was formed by using the multi-layer procedure recommended in the manufacturer’s instructions. Briefly, solution A (175 µL) was placed on the TA layer, and the same volume of solution B was placed on the solution A layer. C After incubating the mixture under ambient conditions for 5 min, the resulting hydrogel was removed with tweezers. D–F Beriplast P prepared by mixing diluted solution A and B was also evaluated. D Just mixing. E After 5 min. F Removing Beriplast P