Figure 5. Protein expression of PD-L1, arginase-1, LOX-1, and CD10 and detection of reactive oxygen species in CD45^high low-density neutrophils and CD45^low low-density neutrophils.

(A) PD-L1 extracellular staining, arginase-1 intracellular staining, and reactive oxygen species detection by an aminophenyl fluorescein fluorescent probe. The horizontal line corresponds to the positivity threshold determined for each stain by its respective Fluorescence Minus One. (B, C, D, E) Percentage of cells above the positivity threshold and (C, E) the increase in mean fluorescence intensity between the stained sample and Fluorescence Minus One. Patient samples used in panels (B, C, D, E) were different. Boxes show the median, and whiskers, the minimal and maximal values. Statistical analyses: paired t test. P-values: ns (non-significant), ≥0.05; *, <0.05; and ****, <0.0001.