Table 5.
Summary of calculated virus reduction factors for affinity chromatography treatment step. (Reduction factors [log10] ± 95% confidence index). Note that two replicates were used for each sample that demonstrated as Run 1 & Run 2.
| Sample 2 | Sample 3 | Sample 4 (TCID50) | Sample 4 (qPCR) | Sample 5 | |
|---|---|---|---|---|---|
| Reovirus | Run 1: 0.25 | Run 1: 1.50 | Run 1: 3.75 | Run 1: 2.81 | Run 1: 3.50 |
| Run 2: 0.50 | Run 2: 1.00 | Run 2: 4.25 | Run 2: 2.80 | Run 2: 3.25 | |
| Average: 0.37 | Average: 1.25 | Average: 4.00 | Average: 2.81 | Average: 3.38 | |
| VSV | Run 1: 0.75 | Run 1: 1.25 | Run 1: 5.00 | Run 1: 2.82 | Run 1: 4.00 |
| Run 2: 0.50 | Run 2: 1.00 | Run 2: 4.50 | Run 2: 3.06 | Run 2: 4.25 | |
| Average: 0.62 | Average: 1.12 | Average: 4.75 | Average: 2.94 | Average: 4.12 | |
| HSV1 | Run 1: 0.50 | Run 1: 0.75 | Run 1: 4.50 | Run 1: 3.05 | Run 1: 5.00 |
| Run 2: 0.50 | Run 2: 1.25 | Run 2: 4.00 | Run 2: 2.90 | Run 2: 4.50 | |
| Average: 0.50 | Average: 1.00 | Average: 4.25 | Average: 2.97 | Average: 4.75 | |
| EMCV | Run 1: 0.25 | Run 1: 1.00 | Run 1: 5.00 | Run 1: 3.03 | Run 1: 4.75 |
| Run 2: 0.75 | Run 2: 0.75 | Run 2: 5.50 | Run 2: 2.80 | Run 2: 5.25 | |
| Average: 0.50 | Average: 0.87 | Average: 5.25 | Average: 2.91 | Average: 5.00 |