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SDS-PAGE separation of culture supernatant samples of secreted protein of B. subtilis VTT E-68013 in different culture media after 50 h of cultivation. Lanes: 1 and 6, molecular weight markers; 2, wheat bran; 3, Luria broth; 4, Luria broth supplemented with 10 mM phytate; 5, Luria broth supplemented with 2% bovine serum albumin; 7, purified recombinant phytase (phytase band indicated by arrow).
