TABLE 2.
N-terminal and internal peptide sequences of PhyC and degenerate primers for PCR designed thereof
| Mol wt of peptide
|
Amino acid sequenceb | Degenerate primer
|
||
|---|---|---|---|---|
| Determineda | Calculated | Nucleotide sequencec | Designation | |
| LSDPYHFTVNAAAETEPVDTAGDAA | TCIGATCCITATCATTTTACIGT | 6465 | ||
| LSDPYHFTVNAAAETEPVDTAGDAADDPAILD | ||||
| 932 | 932.1 | YYAMVTGK | TTTICCIGTIACCATIGC | 6544 |
| 1,271.4 | 1,271.3 | EGEFEQYELK | TTCATA(T/C)TGTTCAAATTCICC | 6472 |
| 1,050.3 | 1,050.2 | MLHSYNTGK | TTICCIGT(A/G)TTATAIGAATGIA(A/G)CAT | 6473 |
| 798.9 | 798.9 | IVPWER | ||
| 2,951.2 | 2,948.4 | IVPWERIADQIGFRPLANEQVDPRK | TGATCIGC(G/A)ATIC(G/T)TTCCCA | 6470 |
| NGTLQSMTDPDHPIATAINEVYGFTLWHSQ | GC(G/A)AT(C/A)GGATGATC(C/A)GGATC | 6471 | ||
| YVADFRITDGPETDGTSDDDGII | TCIGATTCIGGICCATCIGT | 6468 | ||
| 775.7 | 775.8 | LTDRSGK | TTTICCI(G/C)(T/A)IC(G/T)ATCIGT | 6543 |
| 1,317.9 | 1,317.4 | VDIAAASNRSEGK | CTTCIGAIC(G/T)(G/A)TTIGAIGCIGC | 6469 |
| 2,167.4 | 2,167.4 | IADQIGFRPLANEQVDPRK | ||
| 720.7 | 720.8 | ANQNFK | TTTAAA(G/A)TT(C/T)TG(G/A)TTIGC | 6541 |
| 619.6 | 619.7 | VRAFK | ||
| LNNVDIRYDFP | AG(C/A)GGAAAATCATAIC(C/T)(G/A)ATATC | 6467 | ||
| 1,779.4 | 1,778 | LNNVDIRYDFPLNGK | ||
| 1,236.3 | 1,236.4 | NTIEIYAIDGK | CCATC(G/A)ATIGCATA(G/A)ATTTC | 6474 |
| 1,137.4 | 1,137.3 | SGLVVYSLDGK | TTICCATCIA(G/T)I(G/C)(T/A)ATAIAC | 6542 |
| FSAEPDGGSNGTVIDRADGRHL | CCATCIGCIC(G/T)ATC(G/A)ATIAC | 6475 | ||
a
Molecular weight of peptide determined by mass spectrometer.
b
Sequences of LysC-digested purified peptides, the first one being the N-terminal peptide. The region from which the degenerate primer was designed is underlined.
c
Designed degenerate primers, the first one being the forward primer and the others being reverse primers. I, inosine. All primer sequences are written in 5′→3′ direction.