Table 3.
Mutations of the eight flavivirus-conserved amino acids in the WNV EDI-EDII hinge region.
| Location | Amino acid substitutions in the E protein | Nucleotide substitutions in the genome position | ||
|---|---|---|---|---|
| 1st codon | 2nd codon | 3rd codon | ||
| Motif #1 | A54S | G1126T | ||
| Motif #2 | I130N | T1355A | ||
| I135N | T1370A | |||
| Motif #3 | V196T | T1553A | ||
| Y201P | T1567C | A1568C | ||
| Motif #4 | A269S | G1771T | ||
| V272T | G1870A | T1871C | ||
| L281N | T1807A | T1808A | G1809C | |
Each of the eight flavivirus-conserved hydrophobic residues in the WNV EDI-EDII hinge region was replaced with an amino acid of lower hydrophobicity.