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. 2023 Jun 30;129(9):1417–1431. doi: 10.1038/s41416-023-02318-7

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Fig. 2 — Composite spectral mixing image and detail from six marker multiplex immunofluorescence panel using tyramide signal amplification (a), an image showing six important markers extracted from a 26 marker hi-plex methodology using barcoding base-oligonucleotides methodology and detail (b), and seven representative markers from a panel of 95 markers using Imaging Cyclic Staining platform and detail (c). a The image was generated using Vectra-Polaris 1.0.13 scanner system and InForm 2.4.8 image analysis software (Akoya Biosciences), b the image was generated using the PhenoCycler system and the QuPath (open sources), c the image was generated using the MACSima Imaging System and Qi Tissue™ Image Analysis Software (Miltenyi Biotec).