Table 3.

Different scanners used for multiplexed image technologies.

Instrument (company)	Slide loading capacity	Scanning speed	Resolution	Scanning capacity (whole slide WS vs. ROIs)	Multispectral Range
Pannoramic 250 FLASH III (3DHistech, Budapest, Hungary)	250	5 min at 31× 15 min at 62×	0.172 and 0.087/ 0.325 and 0.162 pixels	WS	Undisclosed
Aperio GT 450 (Leica Biosystems, Illinois, USA)	450	32 sec (15 mm × 15 mm area at 40x)	0.26 μm/pixel at 40×	WS	Undisclosed
PhenoImager TH (Akoya Biosciences, Marlborough, MA, USA)	80	Fluorescence WS (1.5 cm × 1.5 cm sample at 20×): 11 slides/hour (7 Colour Opal, unmixed) Bright-field WS (1.5 cm ×  1.5 cm sample at 20X): 10 slides/hour	10 × (1.0 m/pixel), 20 × (0.5 μm/pixel) and 40 × (0.25 µm/pixel)	WS for until 6 markers/ROI for more than 6 markers staining	440–780 nm
NanoZoomer S360 (Hamamatsu, Shizuoka, Japan)	360 (82 slides/h)	Approx. 30 s	20 × (0.46 μm/pixel) 40 × (0.23 μm/pixel)	WS	No fluorescence
Axioscan 7 (Zeiss, Jena, Germany)	100	Brightfield 10 × 10 mm, 20 ×, 1:13 min Fluorescence 10 × 10 mm, 20 × , 4 FL channels (10 ms exposure each) 5:23 min	10 × 0.345 μm/pixel 20 × 0.173 μm/pixel 40 × 0.086 μm/pixel	WS	380 nm – 770 nm
VENTANA DP 600 (Roche/Ventana, Arizona, USA)	240	<49 s at 20× <85 s at 40× (15 mm × 15 mm AOIs)	40 × 0.23 μm/pixel	WS	No fluorescence
MACSima™ Imaging Platform (Miltenyi Biotec, Gaithersburg, USA)	4	Epifluorescence microscope, 30 to 35 min per cycle	2 × 0.06 μm/pixel 20 × 0.75 μm/pixel	WS	386–725
PhenoCicler-Fusion (Akoya Biosciences, Marlborough, MA, USA)	4	Fluorescence, Brightfield. 1 cycle in 10 min	10 × (1.0 m/pixel), 20 × (0.5 μm/pixel) and 40 × (0.25 µm/pixel)	WS	440–780 nm
Orion™ Instrument (RareCyte, INC., Seattle, WA, USA	2	Brightfield, Fluorescence, 75 min per cm2 at 20×	sCMOS 2 K x 2 K pixels 6.5 μm pixel size 95% QE 1.0 electron read noise	WS	400–890 nm
COMETTM (Lunaphore technologies, Tolochenaz, Switzerland)	4	Fluorescence, Average time of 40 min every 2 markers	20 × (0.5 μm/pixel)	WS	Undisclosed