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. 2023 Nov 6;14:7152. doi: 10.1038/s41467-023-42601-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Liposome swelling in the presence of indicated substrate. Liposomes with embedded full-length VpOmpM1, VpOmpM1 barrel-only or EcOmpF were tested. Swelling rates were normalised to the rate of swelling of EcOmpF-containing liposomes in the presence of arabinose. Each condition was measured in technical triplicates (except EcOmpF with fructose, where n = 2) from the same proteoliposome preparation. Error bars show mean ± SD. b Bilayer electrophysiology conductance distribution plots obtained from recordings with multiple insertion events (see Supplementary Fig. 15 for representative traces). Full-length VpOmpM1 distribution is centred on 4.87 ± 1.86 nS (SD, n = 117); VpOmpM1 barrel-only distribution is centred on 4.66 ± 1.88 nS (SD, n = 269). c Representative bilayer electrophysiology recordings of full-length VpOmpM1 (top) showing multiple sub-conductance states and of the barrel-only construct (bottom) showing classical trimeric porin sequential channel closure at high voltage. d Current-voltage characteristics from single-channel recordings of full-length VpOmpM1 (n = 4), barrel-only VpOmpM1 (n = 4), and EcOmpF (n = 3). Individual data points are shown as open black circles and means as coloured dots. The error bars show the SD.