TABLE 3.

Summary of fingerprinting and sequencing results

Bradyrhizobium strain	Single and combined types of 16S rDNA and IGS restriction patternsa			Type of rep-PCR fingerprintb			Combined ARDRA and rep-PCR fingerprint and sequence typec
	CDMM	DHM	CDMM-DHM	BOX	ERIC	REP	BERCDMM	16S
BC-C1	BBAA	3	BBAA-3	U	U	U	U-BBAA	S2
CIAT 3101	BBAA	2	BBAA-2	U	U	U	U-BBAA	S2
USDA 110	BBAB	6	BBAB-6	U	U	U	U-BBAB	S4
BC-P6	BBAB	1	BBAB-1	U	U	U	U-BBAB	S3
BC-P7	BBAB	5	BBAB-5	U	U	U	U-BBAB	S3
BGA-1	BBAB	5	BBAB-5	U	U	U	U-BBAB	S3
BRE-1	BBAB	1	BBAB-1	U	U	U	U-BBAB	S3
BC-C2	AAAB	5	AAAB-5	U	U	U	U-AAAB	S1
BC-P5	AAAB	5	AAAB-5	U	U	U	U-AAAB	S1
BES-1	AAAB	4	AAAB-4	I	I	I	I-AAAB	S1
BTA-1	AAAB	4	AAAB-4	I	I	I	I-AAAB	S1

^aA and B denote the types of restriction pattern for the restriction enzyme as follows. The CDMM column indicates the restriction patterns obtained, respectively, with the tetrameric endonucleases CfoI, DdeI, MboI, and MspI used for ARDRA. The DHM column indicates the types of restriction patterns obtained with DdeI, HaeIII, and MspI, respectively, used in IGS PCR-RFLP analysis. The CDMM-DHM column indicates the genotypes resolved by combined 16S rDNA and 16S-23S rDNA restriction analysis with the enzymes indicated above. The sizes of the resulting restriction fragments in each pattern are listed in Table 2. 

^bU and I stand, respectively, for unique and type I rep-PCR patterns generated with the indicated rep-PCR primers. 

^cLetters in the BERCDMM column indicate the type of combined 16S rDNA restriction patterns and rep-PCR fingerprints. In the 16S column, S1 to S4 are the sequence types obtained for the Canarian and reference bradyrhizobia, as indicated in Fig. 6.