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. 2023 Oct 24;13:1268227. doi: 10.3389/fcimb.2023.1268227

Figure 6.

Figure 6

Cytokine mRNA accumulation in rSARS-CoV-2-D614G infected cells. Calu3 2B4 or Huh-7-ACE2 cells were mock-infected (black) or infected at a MOI of 1 with mutant rSARS-CoV-2-D614G (red) virus. Quantification of mRNAs encoding IFN-β, IFN-Λ, ISG15, MX1, TNF, IL6, CCL2, and CXCL-10 was performed by RT-qPCR using specific TaqMan assays. The HMBS mRNA was used as a reference gene; relative mRNA levels were based on the comparison with mock-infected cells. The data represent the mean from three different infections. Error bars indicate SDs. Compared with mock infected cells, p value, *, <0.05; **, < 0.01, ***, < 0.001.