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. 2023 Oct 21;68:102945. doi: 10.1016/j.redox.2023.102945

Fig. 1.

Fig. 1

c-Met is overexpressed in RCC and a combination of XL-184 and Honokiol (HNK) synergistically restricts renal cancer cell growth. (A) The gene expression profile of c-Met in patient tumor samples and paired normal tissues in different cancer types was evaluated using GEPIA database. c-Met is overexpressed (underlined) in clear cell (KIRC) and papillary (KIRP) type RCC. (B) The body map representing differential median expression of c-Met in normal and various cancer types was analyzed using GEPIA database. (C–D) Gene expression of c-Met in normal and primary tumor samples in (C) human clear cell renal carcinoma (ccRCC), and (D) papillary RCC were analyzed by UALCAN database (left panels). c-Met expression in different stages of (C) ccRCC and (D) papillary RCC were analyzed in a similar way (right panels). ****p < 0.0001. (E) Expression of c-Met in normal RPTEC versus RCC cells (786-0, ACHN, Caki-1 and Caki-2) were analyzed by Western blot. Molecular weight markers (kDa) are on the left. The bar graphs under the Western blots represent the relative expression of c-Met by densitometry, where the signals were normalized to the expression of an internal control, β-Actin. The columns represent the average ± SD of relative intensities (n = 3). Representative of three. ****p < 0.0001 compared with RPTEC. (F) 786-0 and ACHN renal cancer cells were treated with increasing concentrations of XL-184 (1–30 μM), HNK (5–50 μM) or vehicle for 24 h. Control was treated with vehicle alone. Following treatments, cell viability was assessed using CCK-8 assay; the graphs represent percent cell viability of 786-0 (black) and ACHN (red) cells. Representative of three experiments. (G) 786-0 and ACHN cells were treated with combinations of XL-184 (1–5 μM), HNK (5–20 μM) or vehicle alone for 24 h. Following treatments, cell viability was evaluated by CCK-8 assay. Heat maps represent the percent growth inhibition of 786-0 and ACHN cells (left panels) following either individual or combination treatment. Synergy plots (right panels) represent synergism among XL-184 and HNK treatments. Representative of three different experiments. Positive score (red) indicates the synergism, whereas negative score (green) indicates antagonistic effect. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)